P7012

Sigma

 

Pepsin from porcine gastric mucosa

lyophilized powder, 2,500-3,500 units/mg protein (E1%/280)

Synonym:Pepsin A, Pepsin from hog stomach
CAS Number:9001-75-6
Enzyme Commission (EC) Number:3.4.23.1   ( BRENDA | IUBMB )
EC Number:232-629-3
MDL number:MFCD00081840

Description

Analysis NoteOptimum pH is 2-4. Active in 4 M urea and 3 M guanidine HCl. Stable at 60 °C. Pepsin is irreversibly inactivated at pH>6.
 E1%/280=14.7
 Protein determined by E1%/280
ApplicationUsed to produce F(ab′)2 fragments of antibodies.
Biochem/physiol ActionsPreferential cleavage: hydrophobic and aromatic residues in P1 and P1' postitions. Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe and Phe-Tyr bonds in the β chain of insulin
Other NotesView more information on pepsin at www.sigma-aldrich.com/enzymeexplorer.
Unit DefinitionOne unit will produce a ΔA280 of 0.001 per min at pH 2.0 at 37°C, measured as TCA-soluble products using hemoglobin as substrate. (Final volume = 16mL. Light path = 1cm.)

Properties

formlyophilized powder
mol wtmol wt 35 kDa
color off-white to tan
EQP levelPremium
storage temp.−20°C

Safety

Personal Protective Equipmentdust mask type N95 (US), Eyeshields, Faceshields, Gloves
Hazard CodesXn
Risk Statements36/37/38-42
Safety Statements22-24-26-36/37
WGK Germany1
RTECSSC6132000

Related Products

InhibitorP5318, Pepstatin A
 P7424, Pepsinostreptin
 P7626, Phenylmethanesulfonyl fluoride

References

reference, Harlow, E., and Lane, D. Antibodies: A Laboratory Manual Cold Spring Harbor, NY , (1988), 626-628
 Ryle, A.P. Meth. Enzym. Anal. 3rd ed., 5, 228, (1984)
MerckMerck 13,7225